實(shí)驗(yàn)地點(diǎn):蚌埠市刑事警察支隊(duì)
使用儀器:凈信JXFSTPRP-II-01全自動(dòng)液氮冷凍研磨儀
原理:全自動(dòng)樣品冷凍研磨儀采用了特殊的垂直左右8字型震動(dòng)模式,通過(guò)大鋼珠在罐體內(nèi)的高頻往復(fù)振動(dòng)、撞擊、剪切。快速的實(shí)現(xiàn)破碎骨頭、牙齒組織的目的,且通過(guò)液氮降溫,用來(lái)提取組織內(nèi)DNA樣本。
研磨儀優(yōu)點(diǎn):使研磨的樣品具有更加充分、更均勻、一類(lèi)相對(duì)難以研磨的樣本能在短時(shí)間實(shí)現(xiàn)破碎。
實(shí)驗(yàn)步驟:
1、準(zhǔn)備全自動(dòng)液氮快速研磨儀。
2、金屬適配器,以及鋼珠適配器。
3、錘子敲碎取骨骼取部分,放入研磨罐,放入液氮中浸入使之不沸騰。
4、將適配器裝入凈信研磨儀中,倒入液氮保持溫度。
5、設(shè)定參數(shù),啟動(dòng)儀器至研磨程序結(jié)束,可得到如圖所示樣品。
實(shí)驗(yàn)樣品研磨前后:
儀器介紹:全自動(dòng)液氮冷凍研磨機(jī)JXFSTPRP-II-01(專(zhuān)利產(chǎn)品,仿冒必究!2016214095849)
應(yīng)用范圍:該產(chǎn)品專(zhuān)用于研磨熱敏感性物質(zhì)、多種動(dòng)植物組織、微生物、橡膠、塑料、食品、藥品、煤炭、油頁(yè)巖、蠟制品、PE、PS、紡織品、樹(shù)脂等及在常溫下呈韌性、難以粉碎的物質(zhì)。液氮研磨機(jī)就是先把上述物質(zhì)被冷凍到脆化點(diǎn)以下,然后在研磨機(jī)內(nèi)被研磨到所需的細(xì)度,而且原成分不會(huì)破壞。并且在常溫下易燃易爆的物質(zhì),也能低溫粉碎,效果更為顯著。
產(chǎn)品特點(diǎn):
A.?dāng)?shù)據(jù)的準(zhǔn)確性和重現(xiàn)性高
1.研磨過(guò)程中樣品始終處于液氮溫度
2.1分鐘內(nèi)樣品即冷卻到-100℃,4分30秒內(nèi)可達(dá)-196℃
3.樣品瓶在破碎過(guò)程中處于全封閉狀態(tài),樣品完整保留在瓶?jī)?nèi),確保對(duì)危險(xiǎn)、重要樣品的控制
4.樣品瓶均獨(dú)立密封,每次破碎后清洗十分方便,避免樣品間的交叉污染以及外界污染
B.多功能性,人性化設(shè)計(jì)
1.觸摸式顯示屏,可對(duì)研磨時(shí)間、循環(huán)周期、研磨運(yùn)行頻率等參數(shù)進(jìn)行設(shè)置(選配)
2.可移動(dòng)控制面板(選配)
3.可儲(chǔ)存10個(gè)程序(選配)
4.可安1.5ML 2ML 5ML 10ML 25ML 50ML等樣品管,進(jìn)行樣品研磨
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