課題組介紹:張?zhí)煺?,求是特聘教授,博?dǎo),研究方向:作物遺傳育種。主要開展棉花結(jié)構(gòu)基因組、重要性狀基因克隆與功能驗(yàn)證、多倍體遺傳與分子進(jìn)化和生物信息學(xué)等方面研究。
實(shí)驗(yàn)?zāi)康模?/strong>研磨棉花幼苗枝干提取DNA及RNA
實(shí)驗(yàn)地點(diǎn):浙江大學(xué)農(nóng)業(yè)與生物技術(shù)學(xué)院
實(shí)驗(yàn)材料和器具:
樣品:棉花幼苗枝干
儀器:全自動(dòng)低溫冷凍研磨儀(上海凈信JXFSTPRP-CLN)、鋼罐一套
實(shí)驗(yàn)步驟:
1、將棉花幼苗枝干剪成2-5mm長(zhǎng)短的樣品;
2、將處理好的樣品加入鋼罐中;
3、加入配套的研磨珠子一顆,放入液氮種預(yù)冷幾分鐘;
4、將冷凍過的鋼罐樣品裝入凈信研磨儀中;
4、設(shè)定運(yùn)行參數(shù);
5、啟動(dòng)儀器至研磨程序結(jié)束,可得到如圖所示樣品。
實(shí)驗(yàn)結(jié)果:
儀器介紹:
凈信上海凈信JXFSTPRP-CLN冷凍研磨儀是一種快速高效、多試管的一致系統(tǒng)。它能將任何來源(包括土壤、植物和動(dòng)物的組織/器官、細(xì)菌、酵母、真菌、孢子、古生物標(biāo)本等)的原始DNA、RNA和蛋白質(zhì)進(jìn)行提取和純化。廣泛應(yīng)用于生命科學(xué)、醫(yī)藥、生化、環(huán)境、地質(zhì)、農(nóng)業(yè)、林業(yè)、質(zhì)檢、材料等領(lǐng)域。
JXFSTPRP-CLN產(chǎn)品原理:
將樣品和研磨球放入研磨儀內(nèi)(配置研磨罐或離心管/適配器),在高頻擺動(dòng)作用下,研磨球在研磨儀內(nèi)來回高速撞擊及摩擦,在非常短的時(shí)間內(nèi)便可完成樣品的研磨、粉碎、混合及細(xì)胞破壁。
性能特點(diǎn):
1.不破壞樣品成分:研磨溫度可自定義調(diào)節(jié),能夠有效抑制核酸降解,保留蛋白質(zhì)活性;
2.制冷可調(diào):-50至37度
3.高通量處理量:一次處理多達(dá)192個(gè)樣品
4.操作簡(jiǎn)便:內(nèi)置程序控制器,可對(duì)參數(shù)進(jìn)行設(shè)置;
5.安全性高:具備安全罩及安全鎖,無需涉及液氮操作,安全性高;
6.無交叉污染:在研磨過程中處于全封閉狀態(tài),避免交叉污染;
7.低噪音:儀器運(yùn)行過程中,噪音小于55dB,不會(huì)對(duì)其它實(shí)驗(yàn)或儀器產(chǎn)生干擾。
8.無易損件設(shè)計(jì),電磁鎖定保護(hù),不銹鋼內(nèi)腔,清潔消毒方便
9.重復(fù)性穩(wěn)定:相同研磨程序獲得相同效果
10.程序可存儲(chǔ):可存儲(chǔ)10組自定義研磨程序
11.研磨效果好:出料粒度5um
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