實驗目的:研磨魚骨提取鈣鐵鋅硒等微量元素。
實驗地點:中國農(nóng)大實驗老師:趙新剛(研究方向:動物類飼料的營養(yǎng)及吸收)。
實驗前主要用刀片式粉碎機打粉,存在以下問題:
粉碎顆粒大,提取純度及數(shù)量低,影響影響實驗效果。
實驗材料和器具:
樣品:魚骨;
工具:全自動樣品快速研磨儀(JXFSTPRP-24L);
配件:研磨罐、鋼珠若干;
研磨條件:冷凍干磨;
實驗步驟:
1.魚骨切小塊,放入液氮浸泡5分鐘,不銹鋼罐液氮浸泡5分鐘;
2.將冷凍好的魚骨樣品和鋼珠放入研磨罐,并放入適配器中;
3.蓋上蓋板,固定好旋鈕;
4.設定參數(shù),啟動儀器至研磨程序結束;
5.研磨完畢后請關閉電源,將安全鎖歸位。
樣品研磨效果如下:
實驗解決的問題:各樣品之間無污染,微量樣品也能研磨,一次處理的樣品數(shù)量大幅提升,提升工作效率和微量元素的提取率。
注意事項:魚骨在常溫下性質(zhì)比較疏松有彈性,研磨前需將魚骨及研磨罐進行液氮冷凍,使魚骨變得比較脆硬便于研磨。
全自動樣品快速研磨儀圖片
儀器概述:
上海凈信的JXFSTPRP-L系列組織研磨儀(專利產(chǎn)品,仿冒必究!ZL2019301918692)采用進口電機,防腐材質(zhì),增強型運動軸,采用三維一體8字形振蕩方式,研磨更充分,穩(wěn)定性更好。它是一種特殊的、快速的、高效率的、多試管的一致系統(tǒng)。能將任何來源(包括土壤、植物和動物的組織/器官、細菌、酵母、真菌、孢子、古生物標本等)的原始DNA、RNA和蛋白質(zhì)進行提取和純化。?將樣品和研磨球放入研磨儀內(nèi)(配置研磨罐或離心管/適配器),在高頻擺動作用下,研磨球在研磨儀內(nèi)來回高速撞擊及摩擦,在非常短的時間內(nèi)便可完成樣品的研磨、粉碎、混合及細胞破壁。
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