人和動(dòng)物的肝臟具有極強(qiáng)的再生能力,研究人員也通常用大鼠來做實(shí)驗(yàn)從中去研究肝臟的再生能力。通過分離、純化再生肝中各種細(xì)胞,研究大鼠肝再生肝臟細(xì)胞增殖周期變化及體外細(xì)胞增殖周期。
大鼠肝70%切除后可在7-10天內(nèi)基本恢復(fù)原有的體積和功能。從負(fù)調(diào)機(jī)制角度對肝再生進(jìn)行研究,闡明調(diào)控肝再生終止和肝臟組織結(jié)構(gòu)重塑的分子機(jī)制,不僅有助于全面認(rèn)識(shí)肝臟再生,同時(shí)對肝臟疾病,尤其是肝臟腫瘤病理機(jī)制的認(rèn)識(shí)和治療靶點(diǎn)的探索等都具有十分重要的意義。
杭州阿諾生物醫(yī)藥科技有限公司在樣品前處理這塊也在使用我們凈信研磨儀,這次他們的實(shí)驗(yàn)對象是大鼠。
實(shí)驗(yàn)地點(diǎn):阿諾醫(yī)藥
實(shí)驗(yàn)材料和器具:
樣品:大鼠浸泡保存的臟器
儀器:全自動(dòng)快速研磨儀(上海凈信JX-24)
配件:24金屬適配器、氧化鋯珠、氧化鋯珠
JXFSTPRP?系列研磨機(jī)采用了特殊的上下及左右晃動(dòng)三維一體的震動(dòng)模式,樣品在空間呈8字形三維運(yùn)動(dòng),通過研磨珠(氧化鋯、鋼珠、玻璃珠、陶瓷珠)的高頻往復(fù)振動(dòng)、撞擊、剪切??焖俚膶?shí)現(xiàn)目的。使研磨的樣品具有更加充分、更均勻、樣品重復(fù)性更好、樣品之間沒有交叉污染。
實(shí)驗(yàn)步驟:
1、將整塊的大鼠浸泡保存的臟器處理成黃豆大小以備研磨;
2、將樣品加入到無酶離心管中;
3.、加入若干顆氧化鋯珠或氧化鋯珠;
4、設(shè)定運(yùn)行參數(shù)
5、啟動(dòng)儀器至研磨程序結(jié)束,可得到如圖所示樣品。
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