實(shí)驗(yàn)?zāi)康模宏P(guān)于家禽(牛、羊)肌肉中DNA提取
實(shí)驗(yàn)地點(diǎn): 浙江省農(nóng)業(yè)科學(xué)院畜牧獸醫(yī)研究所
實(shí)驗(yàn)材料和器具
樣品:羊的后退肌肉
儀器:全自動(dòng)樣品快速研磨儀(上海凈信Ts-24)、24位金屬適配器
耗材:XXmm鋼珠、XXmm鋼珠、裂解液
實(shí)驗(yàn)步驟
1、將整塊樣品從冰箱中取出,放入液氮中冷凍及分鐘;
2、冷凍過后的樣品用先用硬物敲碎預(yù)處理;
3、將適配器放入液氮中預(yù)冷;
4、設(shè)定好運(yùn)行參數(shù);
5、離心管內(nèi)加入鋼珠幾顆,加入適量樣品和裂解液;
6、迅速將裝有樣品的離心管放入預(yù)冷的適配器中
7、啟動(dòng)儀器至研磨程序結(jié)束,可得到研磨后樣品;
實(shí)驗(yàn)結(jié)果
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