實驗目的:研磨水稻種子更有效快速的提取核酸。
實驗地點:中國科學院植物研究所
實驗材料和器具:
樣品:水稻種子
儀器:全自動快速研磨儀(上海凈信,JXFSTPRP-64)
配件:不銹鋼研磨罐(上海凈信),研磨珠(上海凈信)
研磨條件:干磨液氮預冷處理
實驗步驟
1.將凈信專用研磨罐和研磨球用乙醇清洗干凈,并滅菌滅酶;
2.取適量需要研磨的水稻種子裝入研磨罐中,加入研磨珠,蓋好蓋子;
3.取液氮放在泡沫箱中,將裝好水稻種子的研磨罐置于液氮冷凍幾分鐘。
4.將研磨罐裝入研磨儀。
4.設(shè)定好參數(shù),啟動儀器至研磨程序結(jié)束
需了解具體步驟,請致電廠家
實驗研磨結(jié)果
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