實驗背景
大、小鼠皮膚提蛋白目前是各高校動物實驗室的一項難題,目前客戶用勻漿器手動研磨方法操作麻煩,溫度控制難度大,蛋白容易在研磨過程中因瞬時溫度和研磨顆粒較大,無法做出好的實驗效果。上海凈信研磨儀根據(jù)客戶要求提供技術支持,解決華中科技大學生科院某實驗室技術難題。
某生物實驗交流網(wǎng)站帖子詢問WB解決方案,無準確回答解決方案。
實驗解決方案:
1.儀器配置:上海凈信多樣品組織研磨儀一臺,模塊一套。
研磨儀主機/5ml不銹鋼罐適配器
2.實驗器材需求:適量大小保溫瓶一個,眼科剪,鑷子,鑰勺,后續(xù)配套離心管、裂解液。
皮膚去脂肪層鋪展開效果/ 取材,準備放入適配器液氮冷凍
將皮膚放入5ml不銹鋼罐,剪碎至一定程度,放入鋼珠,然后一同放入保溫瓶中冷凍至不沸騰,大概3分鐘左右將適配器一同快速裝入研磨機中,調(diào)好頻率和時間研磨。研磨完成后將組織轉入EP管中用裂解液溶解。
皮膚研磨后效果圖/加入裂解液后效果圖
研磨完成后進行體蛋白提取及后續(xù)實驗,此方法最小通量為24個2mlEP管。大大提高了實驗人員的工作效率,并且研磨時間短并且均衡,能控制好全程溫度在-80℃以下。
總結:上海凈信研磨儀對皮膚及動、植物或各種材料標本有全套解決方案,高通量地解決實驗重難點問題,讓實驗更加輕松快捷,省去大量人力物力,深受各高校喜愛。
Previous:多樣品組織研磨儀研磨豬皮實驗-上海凈信
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