近年來(lái),隨著生命科學(xué)研究的發(fā)展,尤其是在完成擬南芥 (Arabidopsis thaliana) 和水稻 (Oryza sativa) 等植物的基因組測(cè)序后,植物生物學(xué)發(fā)生了翻天覆地的變化。人們已經(jīng)把目光從基因的測(cè)序轉(zhuǎn)移到了基因的功能研究。在研究 DNA 的基因組學(xué)、mRNA 的轉(zhuǎn)錄組學(xué)及蛋白質(zhì)的蛋白組學(xué)后,接踵而來(lái)的是研究代謝物的代謝組學(xué) (Hall et al.,2002)。代謝組學(xué)的概念來(lái)源于代謝組,代謝組是指某一生物或細(xì)胞在一特定生理時(shí)期內(nèi)所有的低分子量代謝產(chǎn)物,代謝組學(xué)則是對(duì)某一生物或細(xì)胞在一特定生理時(shí)期內(nèi)所有低分子量代謝產(chǎn)物同時(shí)進(jìn)行定性和定量分析的一門新學(xué)科 (Goodacre,2004)。它是以組群指標(biāo)分析為基礎(chǔ),以高通量檢測(cè)和數(shù)據(jù)處理為手段,以信息建模與系統(tǒng)整合為目標(biāo)的系統(tǒng)生物學(xué)的一個(gè)分支。
實(shí)驗(yàn)步驟:
步驟1:將采集的各種植物組織(根莖葉,根瘤菌等)裝入研磨管,加入研磨珠。放入液氮中進(jìn)行低溫處理。
步驟2:將低溫處理過(guò)的樣品放入凈信研磨儀,設(shè)置頻率,研磨完成后可以得到粉末狀的樣品
步驟3:從凈信研磨儀中取出樣品,將樣品溶解做后續(xù)實(shí)驗(yàn)
實(shí)驗(yàn)結(jié)果
研磨效果
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