實驗?zāi)康?/strong>
研磨破碎軟骨組織(大鼠膝關(guān)節(jié)軟骨),提取其總RNA。
實驗原理
充分磨碎軟骨樣品(大鼠膝關(guān)節(jié)軟骨),再用相關(guān)試劑提取其總RNA。
實驗材料和器具
樣品:大鼠膝關(guān)節(jié)軟骨
儀器:多樣品組織研磨儀(上海凈信,Tissuelyser-24)
耗材:研磨罐,研磨珠(上海凈信)
實驗步驟
需了解具體步驟,請致電廠家
實驗結(jié)果
采用本實驗方法提取的動物關(guān)節(jié)軟骨組織總RNA的收率高,并且純度高、質(zhì)量、完整性好,可以滿足分子生物學(xué)研究應(yīng)用。
總RNA甲醛變性膠電泳結(jié)果
Ⅱ型膠原PCR擴增產(chǎn)物電泳結(jié)果
參考文獻:
[1]劉玉剛等,一種富含多糖的關(guān)節(jié)軟骨組織總RNA提取方法初探,第三軍醫(yī)大學(xué)學(xué)報:2009年24期。
Previous:高通量組織研磨儀針對果蠅RNA提取的研磨方法
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