實(shí)驗(yàn)?zāi)康?/strong>
農(nóng)產(chǎn)品及種子檢驗(yàn),經(jīng)常需要對(duì)將植物種子進(jìn)行研磨以獲得DNA、RNA或蛋白質(zhì)等生物分子,從而進(jìn)行后續(xù)的分析和檢測(cè)。隨著實(shí)驗(yàn)室科研進(jìn)度的日益加快,手動(dòng)研磨已無(wú)法滿足科研工作的需要。
本實(shí)驗(yàn)通過(guò)高通量研磨破碎經(jīng)濟(jì)作物種子,進(jìn)而提取其核酸/蛋白質(zhì)進(jìn)行種子檢驗(yàn)和分析。
實(shí)驗(yàn)材料和器具
樣品:水稻,小麥種子
儀器:多樣品組織研磨儀(上海凈信,Tissuelyser-24)
耗材:離心管,研磨珠(上海凈信)
實(shí)驗(yàn)步驟
需了解具體步驟,請(qǐng)致電廠家
注意事項(xiàng)
1. 離心管和研磨珠需充分清洗干凈并烘干備用;若需提取RNA,則研磨罐和研磨珠要確保滅酶;
2. 每個(gè)離心管研磨樣品量一般遵循原則:樣品體積不超過(guò)離心管容積的三分之一。
研磨結(jié)果
Previous:高通量組織研磨儀對(duì)動(dòng)物器官組織的研磨
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