條形酵母是將酵母通過小孔注入到裝有液體氮的氣罐中壓縮制成的。將有篩子的扣入到裝有球狀酵母的破碎罐的頂部,經過離心破碎變成了條狀物。壓力是由人工用自由活塞注射器注入罐內,條狀可以在-80C°下被保存。
使用上海凈信Tissuelyser全自動樣品快速研磨儀對酵母進行研磨,將不銹鋼鋼珠和樣本分別裝入研磨鋼罐中,然后放入到用聚苯乙烯盒子盛放的液氮中,當預冷凍過程結束后,時間一分鐘左右,或者直到原來沸騰的液體氮恢復平靜,將破碎罐從液氮中取出,并要將破碎罐沾有的液體全部清理干凈,以避免在破碎過程中發(fā)生爆炸。研磨鋼罐最好用坩堝鉗或大鑷子夾取出來,而且通常要帶經過冷凍的手套,凍傷以免。
研磨鋼罐中酵母量不要太多,否則可能會研磨不充分,留有殘渣,一般為研磨鋼罐的1/3左右為宜,把剛從液氮中取出的鋼罐安裝在凈信Tissuelyser全自動樣本快速研磨儀中,分幾次去進行研磨,去設置頻率和時間參數(shù),每次間隔都把研磨罐放入液氮中冷卻。
在每次破碎后都要將破碎罐取下,放入到液氮中進行冷凍,當液氮不在沸騰時,可以將鋼罐取出,破碎可以繼續(xù)進行。
經過100秒的破碎后,我們通過顯微鏡觀察可以發(fā)現(xiàn)95%的酵母已經達到破碎效果,用于下一步的實驗過程。剩余的酵母可以儲存,備用。
研磨鋼罐和鋼珠都可重復利用,破碎罐和破碎球可以用溫的乙醇清洗,放到干燥處貯存。
上海凈信實業(yè)發(fā)展有限公司,是目前國內唯一家生產水平式組織研磨機,垂直式組織研磨機,三維一體式組織研磨機具有全系列生產線的生產廠家。同時還給多家國外品牌及國內企業(yè)提供OEM,貼牌代加工服務。
中國多樣品/高通量研磨機廠家!
凈信,就是研磨機!?。?/strong>
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