上海凈信實(shí)業(yè)發(fā)展有限公司,是目前國(guó)內(nèi)一家生產(chǎn)水平式組織研磨機(jī),垂直式組織研磨機(jī),三維一體式組織研磨機(jī)具有全系列生產(chǎn)線(xiàn)的生產(chǎn)廠家。同時(shí)還給多家國(guó)外品牌及國(guó)內(nèi)企業(yè)提供OEM,貼牌代加工服務(wù)。
Tissuelyser系列多樣品組織研磨儀具有高速大振幅的中心軸,通過(guò)研磨珠在樣品管內(nèi)來(lái)回不規(guī)則撞擊及摩擦,形成空間三維立體的碰撞,保證了研磨珠于樣品的充分接觸。在幾十秒到一分鐘內(nèi)輕松實(shí)現(xiàn)樣品的研磨、粉碎、混合及細(xì)胞破壁。精細(xì)的研磨,最細(xì)可以達(dá)到幾微米。
對(duì)于不同的的樣品,Tissuelyser系列多樣品組織研磨儀需要用不同的條件來(lái)處理,這里面主要涉及研磨頻率、研磨時(shí)間、研磨溫度和研磨珠的選擇。以下是Tissuelyser系列多樣品組織研磨儀針對(duì)一些典型樣品的實(shí)驗(yàn)方法,僅供參考
實(shí)驗(yàn)一 昆蟲(chóng)動(dòng)物組織研磨
實(shí)驗(yàn)地點(diǎn):中國(guó)農(nóng)科院蠶業(yè)研究所
實(shí)驗(yàn)材料: 蠶蛾、蠶蛹組織
1 取蠶蛾,剪碎,取其身體組織一塊放置于離心管(EP管)中
2 將管中加入幾顆凈信研磨珠
3 將加完樣品和研磨珠的EP管,在適配器上擺好,蓋上管蓋,整體放入到液氮中進(jìn)行冷凍幾分鐘或直到液氮不沸騰為止。
4 迅速將適配器安置到研磨儀上。
5 設(shè)置研磨儀的時(shí)間和頻率參數(shù),可得到細(xì)碎的樣品粉末,用于提核酸或蛋白。(對(duì)蠶蛹,研磨時(shí)間可適當(dāng)增加,效果也非常好!)
同類(lèi)實(shí)驗(yàn)對(duì)象:以昆蟲(chóng)作為研究材料;需要高通量提取核酸、蛋白進(jìn)行遺傳或是生理生化分析研究的實(shí)驗(yàn)
實(shí)驗(yàn)二 哺乳動(dòng)物組織研磨
實(shí)驗(yàn)地點(diǎn):南方醫(yī)科大學(xué)細(xì)胞生物學(xué)重點(diǎn)實(shí)驗(yàn)室
實(shí)驗(yàn)樣品:小鼠 心臟,脾臟,肝臟組織(動(dòng)物內(nèi)臟組織核酸提?。?/p>
1、取冷凍過(guò)小鼠組織,直徑不要過(guò)大,將其放入圓底離心管中
2、在離心管中加入PBS裂解液;幾分鐘后,待組織塊在裂解液中解凍
3、加入凈信研磨珠幾顆
4、設(shè)置Tissuelyser系列動(dòng)物組織研磨儀的研磨時(shí)間和研磨頻率
實(shí)驗(yàn)結(jié)果3種組織已經(jīng)完全被打碎,三種組織裂解都比較徹底,無(wú)明顯的組織碎片。
同類(lèi)實(shí)驗(yàn)對(duì)象:
以哺乳動(dòng)物內(nèi)臟組織為處理樣品;樣品量小,通量高。需要從內(nèi)臟組織中提取核酸物質(zhì)、或進(jìn)行理化成分分析的實(shí)驗(yàn)。
Tissuelyser系列動(dòng)物組織研磨儀是名副其實(shí)的實(shí)驗(yàn)室樣品制備的多面手,它可以對(duì)硬性、軟性、彈性等樣品進(jìn)行快速的粉碎和均相化處理,符合理化分析實(shí)驗(yàn)室的要求,配置不同體積、不同材料的適配器和研磨罐,可以進(jìn)行干磨、濕磨及冷凍研磨,也可以進(jìn)行細(xì)胞破碎和DNA/RNA提取,在生物醫(yī)藥、農(nóng)業(yè)、食品、科研、檢測(cè)等領(lǐng)域應(yīng)用廣泛。
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